Reference analytics using HPLC Chromatographic methods, such as high-performance liquid chromatography, are technologies with a long tradition. Official methods are mostly based on this technology and they are recognised as a reference. They can be used more universally than methods that lack a chromatographic separation, as they are less influenced by interferences before detection due to the separation. High performance liquid chromatography is mostly used in the field of mycotoxin determination, but gas chromatography is also applied. Thin layer chromatography that is still used for analyzing mycotoxins is an easier and cheaper way to perform liquid chromatography. High performance liquid chromatography for mycotoxins is often connected to variable wavelength detectors or fluorescence detectors. Using these two detectors most of the core mycotoxins can be analysed with sufficient sensitivity. Over the past years great progress has been made in mass spectrometry coupled to high performance liquid chromatography (LCMS) leading to increasing distribution of these instruments. LCMS offers increased possibilities regarding identification and quantification of an increasing number of analytes at lower costs.

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